MassPREP Protein Standard Mix [186004900]

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MassPREP Protein Standard Mix [186004900]

The MassPREP™ Protein Standard Mix is designed for verification of HPLC/UPLC® instruments and performance of columns used for protein analysis, specfically the ACQUITY UPLC® and XBridge® BEH300 C4 Columns. This intact protein validation mix should be used on a regular basis for monitoring column and system performance. The standard is contains ribonuclease A, cytochrome c, bovine serum albumin, myoglobin, enolase, and phosphorylase B, which have a wide range of isoelectric points, molecular weights, and hydrophobicities.

The standard mixture is packaged in a Waters TruView™ Vial (ideal for peptides) as a lyophilized solid containing approximately 123 µg total protein. Refer to the Certificate of Analysis for exact amounts of each protein. Recommended solubilization is in 100 µl of 0.1% trifluoroacetic acid in DI water.

Store at -20 °C immediately upon arrival.

  • ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 2.1 mm X 100 mm, 1/pk (186004496)
  • XBridge Protein BEH C4 Column, 300Å, 3.5 µm, 2.1 mm X 100 mm, 10K - 500K, 1/pk (186004499)
  • TruView™ LCMS Certified Vials (186005666CV)

System Performance Standards Key

Set Up

Setup standards are used upon installation of a new system, start-up of a system which has been idle for a period of time, or to re-start a system that has been moved to a new location. These standards may include multiple components within a kit which allow step by step setup and calibration, or they can be single mixtures which will enable a full system assessment and initialization.


These standards can be used for instrument calibration. Calibration is usually performed at a frequency that minimizes the risk of being out of calibration, in addition to major events suc h as when the instrument is moved and upon any major repair. Calibration can be confirmed as often as your standard operating procedures recommend. Look for solutions to calibrate your MS mass accuracy and resolution, UV detector linearity, UV and Fluorescence detector wavelength accuracy, injector accuracy and precision, and solvent system flow rate accuracy.


Your system is comprised of many interdependent components working together. An issue with any one component can produce erroneous final results. All components performing correctly will produce results within an expected variability. Some of the system components include mobile phase, column, tubing, pumping, injecting, temperature controlling, detecting, data collection rate, and integration
parameters. An issue in any one of the components listed can affect your result.
Performance Check Standards should be utilized on a routine basis. This includes anytime a column is changed, a method is changed, the instrument is moved, or confirmation of system performance is desired. Confirming the performance of your system can be done on a system basis with suitability standards or per individual instrument component. Benchmark performance should be established after
calibration and confirmed regularly.


These Mass Spectrometry solutions are used routinely and are available for every ionization mode. Use the solution to optimize the mass spectrometer setting for optimal signal either manually or through Intellistart.


This product contains hazardous material which requires special freight handling. Additional charges may apply.

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