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Cytochrome C Digestion Standard [186006371]

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Cytochrome C Digestion Standard [186006371]

The Cytochrome C Digestion Standard is part of a line of qualitative digestion standards used for performance evaluation of HPLC and UPLC Columns for peptide separation. This standard is used to benchmark the ACQUITY UPLC and XBridge BEH 300 and 130 C18 as well as the ACQUITY UPLC CSH and XSelect Column lines.

This standard should be used on a regular basis for evaluation of column and system performance. Each digestion standard is batch manufactured with high-grade quality digestion materials and enzymes and is subsequently purified and quality control tested to ensure intra-lot reproducibility.

The Cytochrome C Digestion Standard is prepared by digesting Bovine Heart Cytochrome C (Uniprot # P62894) with sequencing grade trypsin. It is packaged in a TruView Vial (ideal for peptides) and contains 13 nmol of lyophilized solid ready for solubilization and direct injection.

Recommended solubilization for HPLC is in 200 µL of 0.055% trifluoroacetic acid or Mobile phase A.

Store at room temperature.

  • XSelect Peptide CSH C18 XP Column, 130Å, 2.5 µm, 4.6 mm X 150 mm, 1/pk (186007038)
  • ACQUITY UPLC Peptide CSH C18 Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1/pk (186006938)
  • XSelect Peptide CSH C18 XP Column, 130Å, 2.5 µm, 2.1 mm X 150 mm, 1/pkg  (186006943)
  • TruView LCMS Certified Vials (186005666CV)

System Performance Standards Key

Set Up

Setup standards are used upon installation of a new system, start-up of a system which has been idle for a period of time, or to re-start a system that has been moved to a new location. These standards may include multiple components within a kit which allow step by step setup and calibration, or they can be single mixtures which will enable a full system assessment and initialization.

Calibration

These standards can be used for instrument calibration. Calibration is usually performed at a frequency that minimizes the risk of being out of calibration, in addition to major events suc h as when the instrument is moved and upon any major repair. Calibration can be confirmed as often as your standard operating procedures recommend. Look for solutions to calibrate your MS mass accuracy and resolution, UV detector linearity, UV and Fluorescence detector wavelength accuracy, injector accuracy and precision, and solvent system flow rate accuracy.

Performance

Your system is comprised of many interdependent components working together. An issue with any one component can produce erroneous final results. All components performing correctly will produce results within an expected variability. Some of the system components include mobile phase, column, tubing, pumping, injecting, temperature controlling, detecting, data collection rate, and integration
parameters. An issue in any one of the components listed can affect your result.
 
Performance Check Standards should be utilized on a routine basis. This includes anytime a column is changed, a method is changed, the instrument is moved, or confirmation of system performance is desired. Confirming the performance of your system can be done on a system basis with suitability standards or per individual instrument component. Benchmark performance should be established after
calibration and confirmed regularly.

Tuning

These Mass Spectrometry solutions are used routinely and are available for every ionization mode. Use the solution to optimize the mass spectrometer setting for optimal signal either manually or through Intellistart.

Hazardous

This product contains hazardous material which requires special freight handling. Additional charges may apply.

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