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SILAC Hi3 Phos B Standard [186007083]

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SILAC Hi3 Phos B Standard [186007083]

SILAC has emerged as a very powerful method to study cell signaling, post translational modifications, protein-protein interactions, and regulation of gene expression. The quantitative SILAC Hi3 standards can be valuable in performing relative protein quantitation of mixed proteome samples, minimize interferences with the endogenous peptides and they add a second level of internal quality control. The SILAC Hi3 Phos B standard is formulated from the same specialized set of the top 6 ionizing peptides of the rabbit Phosphorylase B protein that is contained in the non-labeled counterpart: Hi3 Phos B standard. The main difference is that this standard is produced to have a heavy labeled reference on the lysine (K) or arginine (R) end of the peptide. The peptides are synthetically prepared and highly purified. They are quantitated via AAA to 1 nmol/vial equimolar ratios to provide greater accuracy to each analysis. They are greater than 95% pure and have been verified by mass identification.

They are packaged in a Waters vial for direct solubilization and injection and come as a lyophilized solid. Recommended solubilization is in 1000 µl of 3% acetonitrile w/ 0.1% trifluoroacetic acid in DI water for 1 pmol/ul, then furthe diluted to 25 fmol/ul.

Store at -20 °C immediately upon arrival.

System Performance Standards Key

Set Up

Setup standards are used upon installation of a new system, start-up of a system which has been idle for a period of time, or to re-start a system that has been moved to a new location. These standards may include multiple components within a kit which allow step by step setup and calibration, or they can be single mixtures which will enable a full system assessment and initialization.

Calibration

These standards can be used for instrument calibration. Calibration is usually performed at a frequency that minimizes the risk of being out of calibration, in addition to major events suc h as when the instrument is moved and upon any major repair. Calibration can be confirmed as often as your standard operating procedures recommend. Look for solutions to calibrate your MS mass accuracy and resolution, UV detector linearity, UV and Fluorescence detector wavelength accuracy, injector accuracy and precision, and solvent system flow rate accuracy.

Performance

Your system is comprised of many interdependent components working together. An issue with any one component can produce erroneous final results. All components performing correctly will produce results within an expected variability. Some of the system components include mobile phase, column, tubing, pumping, injecting, temperature controlling, detecting, data collection rate, and integration
parameters. An issue in any one of the components listed can affect your result.
 
Performance Check Standards should be utilized on a routine basis. This includes anytime a column is changed, a method is changed, the instrument is moved, or confirmation of system performance is desired. Confirming the performance of your system can be done on a system basis with suitability standards or per individual instrument component. Benchmark performance should be established after
calibration and confirmed regularly.

Tuning

These Mass Spectrometry solutions are used routinely and are available for every ionization mode. Use the solution to optimize the mass spectrometer setting for optimal signal either manually or through Intellistart.

Hazardous

This product contains hazardous material which requires special freight handling. Additional charges may apply.

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