HPLC with UV Standards Kit [700003741]

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HPLC with UV Standards Kit [700003741]

The HPLC with UV Standards Kit is intended to be used in the performance qualification of an Alliance® 2695 system with UV or PDA detection. This kit is designed for use by a Waters certified engineer or user as part of a performance maintenance or qualification activity. Protocols in the use of this kit are available via the purchase of Waters qualification services or self directed qualification training and license.

The kit includes 10 Ampules with 10 ml of standard solutions each including:

  • Solution 1, of 0.030 mg/mL Uracil with 0.0100 mg/mL Caffeine
  • Solution 2 of 0.030 mg/mL Uracil with 0.0350 mg/mL Caffeine
  • Solution 3 Qty 2 of 0.030 mg/mL Uracil with 0.0600 mg/mL Caffeine
  • Solution 4 of 0.030 mg/mL Uracil with 0.1400 mg/mL Caffeine
  • Solution 5 of 0.030 mg/mL Uracil with 0.2200 mg/mL Caffeine
  • Solution 6 of 0.010 mg/mL Uracil
  • Solution 7 of 0.40 ug/mL Caffeine
  • Solution 8 of 4.00 mg/mL Caffeine
  • Solution 9 of Blank (mobile phase only)


All standards are in 30% Methanol and 70% Water, V/V.

Store at room temperature.

  • XSelect® CSH C18 XP Column, 130Å, 2.5 µm, 4.6 mm X 100 mm (186006111)
  • LCMS Certified Vials (600000668CV)
  • TruView™ LCMS Certified Vials (186005666CV)

System Performance Standards Key

Set Up

Setup standards are used upon installation of a new system, start-up of a system which has been idle for a period of time, or to re-start a system that has been moved to a new location. These standards may include multiple components within a kit which allow step by step setup and calibration, or they can be single mixtures which will enable a full system assessment and initialization.


These standards can be used for instrument calibration. Calibration is usually performed at a frequency that minimizes the risk of being out of calibration, in addition to major events suc h as when the instrument is moved and upon any major repair. Calibration can be confirmed as often as your standard operating procedures recommend. Look for solutions to calibrate your MS mass accuracy and resolution, UV detector linearity, UV and Fluorescence detector wavelength accuracy, injector accuracy and precision, and solvent system flow rate accuracy.


Your system is comprised of many interdependent components working together. An issue with any one component can produce erroneous final results. All components performing correctly will produce results within an expected variability. Some of the system components include mobile phase, column, tubing, pumping, injecting, temperature controlling, detecting, data collection rate, and integration
parameters. An issue in any one of the components listed can affect your result.
Performance Check Standards should be utilized on a routine basis. This includes anytime a column is changed, a method is changed, the instrument is moved, or confirmation of system performance is desired. Confirming the performance of your system can be done on a system basis with suitability standards or per individual instrument component. Benchmark performance should be established after
calibration and confirmed regularly.


These Mass Spectrometry solutions are used routinely and are available for every ionization mode. Use the solution to optimize the mass spectrometer setting for optimal signal either manually or through Intellistart.


This product contains hazardous material which requires special freight handling. Additional charges may apply.

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