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GlycoWorks RapiFluor-MS N-Glycan Kit Starter Kit [176003635]

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GlycoWorks RapiFluor-MS N-Glycan Kit Starter Kit [176003635]

Re-imagine a streamlined, fast, and highly sensitive way to analyze released N-glycans. With just 3 easy steps in 30 minutes, the GlycoWorks RapiFluor-MS N-Glycan Kit reduces complicated, time-consuming sample preparation. RapiFluor-MS’s unique chemical attributes provide increased fluorescence quantification and supreme mass spectral response. Now you can use a single label that provides valuable information from characterization to routine monitoring. Glycan analysis just got easier and more sensitive. This starter hit includes the GlycoWorks Deglycosylation Module, GlycoWorks Labeling Module, GlycoWorks Clean-up Module, GlycoWorks Sample Collection Module, as well as the ACQUITY UPLC Glycan BEH Amide, 1.7 μm, 2.1 x 150 Column and Mobile-phase Concentrate: Ammonium Formate.

 

 

The kit contains:

GlycoWorks Rapid PNGase F and Buffer:
(4) 0.03 ml/vial Rapid PNGaseF Buffer
(4) 0.25 ml/vial Glycoworks Rapid Buffer
(1) Intact mAb Mass Check Standard 
(4) 10 mg/vial RapiGest SF Surfactant 

GlycoWorks RapiFluor-MS Labeling Module:
(4) 23 mg/vial RapiFluor-MS Reagent
(4) 3 ml/vial Reagent Solvent  (Anhydrous DMF)

GlycoWorks SPE Reagents:
(4) 0.5 ml/vial Elution Buffer (200mM Ammonium Acetate 95/5 Water/ACN pH 7)
(4) 3 ml/vial Sample Diluent (Anhydrous DMF)

(2) GlycoWorks HILIC μElution Plate

GlycoWorks Sample Collection Module:
(15) 1 mL tubes for deglycosylation and labeling reaction
(15) 600 μL tube inserts for SPE eluate collection
(15) Cap strips with slits for direct injection
(1) 1 mL round collection tray
(1) Waste tray

(1) 50 mM Ammonium Formate Solution—Glycan Analysis 

(1) ACQUITY UPLC Glycan BEH Amide Column 
 

  • ACQUITY UPLC Glycan BEH Amide 130Å, 1.7 μm, 2.1 x 50 mm (p/n 186004740)
  • ACQUITY UPLC Glycan BEH Amide 130Å, 1.7 μm, 2.1 x 150 mm (p/n 186004742)
  • XBridge Glycan BEH Amide XP, 130Å, 2.5 μm, 2.1 x 50 mm (p/n 186007263)
  • XBridge Glycan BEH Amide XP, 130Å, 2.5 μm, 2.1 x 150 mm (p/n 186007265)

System Performance Standards Key

Set Up

Setup standards are used upon installation of a new system, start-up of a system which has been idle for a period of time, or to re-start a system that has been moved to a new location. These standards may include multiple components within a kit which allow step by step setup and calibration, or they can be single mixtures which will enable a full system assessment and initialization.

Calibration

These standards can be used for instrument calibration. Calibration is usually performed at a frequency that minimizes the risk of being out of calibration, in addition to major events suc h as when the instrument is moved and upon any major repair. Calibration can be confirmed as often as your standard operating procedures recommend. Look for solutions to calibrate your MS mass accuracy and resolution, UV detector linearity, UV and Fluorescence detector wavelength accuracy, injector accuracy and precision, and solvent system flow rate accuracy.

Performance

Your system is comprised of many interdependent components working together. An issue with any one component can produce erroneous final results. All components performing correctly will produce results within an expected variability. Some of the system components include mobile phase, column, tubing, pumping, injecting, temperature controlling, detecting, data collection rate, and integration
parameters. An issue in any one of the components listed can affect your result.
 
Performance Check Standards should be utilized on a routine basis. This includes anytime a column is changed, a method is changed, the instrument is moved, or confirmation of system performance is desired. Confirming the performance of your system can be done on a system basis with suitability standards or per individual instrument component. Benchmark performance should be established after
calibration and confirmed regularly.

Tuning

These Mass Spectrometry solutions are used routinely and are available for every ionization mode. Use the solution to optimize the mass spectrometer setting for optimal signal either manually or through Intellistart.

Hazardous

This product contains hazardous material which requires special freight handling. Additional charges may apply.

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