MassPREP Phosphorylase b Standard [186002326]

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MassPREP Phosphorylase b Standard [186002326]

The MassPREP™ Phosphorylase b Digestion Standard is part of a line of qualitative digestion standards used for performance evaluation of HPLC, UPLC® Columns and LC-MS instrumentation. It is especially useful in positive ion mode evaluations for sensitivity, resolution, and mass measurement accuracy on nanoACQUITY® and Q-Tof™ based systems.

Each digestion standard is batch manufactured with high-grade quality digestion materials and enzymes and is subsequently purified and quality control tested to ensure intra-lot reproducibility. The resulting protein digest contains no undigested protein, trypsin, or other hydrophilic components.

The Phosphorylase b Digestion Std is derived from rabbit (SwissProt P00489) and is reduced, alkylated and digested with sequencing grade trypsin. It is packaged in a Waters TruView™ Vial (ideal for peptides) and contains 1 nmol of lyophilized solid, ready for solubilization and direct injection. Recommended solubilization for LC is 100 µl of 0.1% trifluoroacetic or formic acid in DI water.

Store at room temperature.


System Performance Standards Key

Set Up

Setup standards are used upon installation of a new system, start-up of a system which has been idle for a period of time, or to re-start a system that has been moved to a new location. These standards may include multiple components within a kit which allow step by step setup and calibration, or they can be single mixtures which will enable a full system assessment and initialization.


These standards can be used for instrument calibration. Calibration is usually performed at a frequency that minimizes the risk of being out of calibration, in addition to major events suc h as when the instrument is moved and upon any major repair. Calibration can be confirmed as often as your standard operating procedures recommend. Look for solutions to calibrate your MS mass accuracy and resolution, UV detector linearity, UV and Fluorescence detector wavelength accuracy, injector accuracy and precision, and solvent system flow rate accuracy.


Your system is comprised of many interdependent components working together. An issue with any one component can produce erroneous final results. All components performing correctly will produce results within an expected variability. Some of the system components include mobile phase, column, tubing, pumping, injecting, temperature controlling, detecting, data collection rate, and integration
parameters. An issue in any one of the components listed can affect your result.
Performance Check Standards should be utilized on a routine basis. This includes anytime a column is changed, a method is changed, the instrument is moved, or confirmation of system performance is desired. Confirming the performance of your system can be done on a system basis with suitability standards or per individual instrument component. Benchmark performance should be established after
calibration and confirmed regularly.


These Mass Spectrometry solutions are used routinely and are available for every ionization mode. Use the solution to optimize the mass spectrometer setting for optimal signal either manually or through Intellistart.


This product contains hazardous material which requires special freight handling. Additional charges may apply.

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