Hi3 Phos B Standard [186006011]

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Hi3 Phos B Standard [186006011]

The Hi3 Phos B standard is a quantitative standard primarily intended for use with the Hi3 quantification method for MSE proteomics data processed with ProteinLynx Global Server for samples of microbial origin. It may also used in the evaluation and benchmarking of proteomic LC-MS systems comprised of nanoACQUITY® UPLC® and Synapt® and Xevo® time-of-flight mass spectrometers.

The Hi3 Phos B standard is intended for samples of microbial origin. It is comprised of the top 6 ionizing peptides in the rabbit Phosphorylase B protein.

This standard is quantitated via amino acid analysis to equimolar ratios to provide greater accuracy to each analysis. It contains synthetic peptides that are greater than 95% pure and have been verified by mass identification. The standard is packaged in a Waters vial and contains 1 nmol of each of the 6 peptides in a lyophilized powder. Recommended solubilization is in 1000 µl of 3% acetonitrile w/ 0.1% trifluoroacetic acid in DI water for 1 pmol/µl, then further diluted to 25 fmol/µl.

Long term storage is recommended at -20 °C.

System Performance Standards Key

Set Up

Setup standards are used upon installation of a new system, start-up of a system which has been idle for a period of time, or to re-start a system that has been moved to a new location. These standards may include multiple components within a kit which allow step by step setup and calibration, or they can be single mixtures which will enable a full system assessment and initialization.


These standards can be used for instrument calibration. Calibration is usually performed at a frequency that minimizes the risk of being out of calibration, in addition to major events suc h as when the instrument is moved and upon any major repair. Calibration can be confirmed as often as your standard operating procedures recommend. Look for solutions to calibrate your MS mass accuracy and resolution, UV detector linearity, UV and Fluorescence detector wavelength accuracy, injector accuracy and precision, and solvent system flow rate accuracy.


Your system is comprised of many interdependent components working together. An issue with any one component can produce erroneous final results. All components performing correctly will produce results within an expected variability. Some of the system components include mobile phase, column, tubing, pumping, injecting, temperature controlling, detecting, data collection rate, and integration
parameters. An issue in any one of the components listed can affect your result.
Performance Check Standards should be utilized on a routine basis. This includes anytime a column is changed, a method is changed, the instrument is moved, or confirmation of system performance is desired. Confirming the performance of your system can be done on a system basis with suitability standards or per individual instrument component. Benchmark performance should be established after
calibration and confirmed regularly.


These Mass Spectrometry solutions are used routinely and are available for every ionization mode. Use the solution to optimize the mass spectrometer setting for optimal signal either manually or through Intellistart.


This product contains hazardous material which requires special freight handling. Additional charges may apply.

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