HIC Protein Standard Mix [186007953]

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HIC Protein Standard Mix [186007953]

The HIC Protein Standard Mix is used to evaluate and benchmark HIC columns, especially the Waters Protein-Pak™ Hi Res HIC Columns.  It is recommended that this standard be used on a regular basis for evaluation of column and system performance ensuring confidence in results.   It is also very valuable for method development and troubleshooting. This standard is uniquely formulated and optimized with a 6-component intact protein mixture to span a typical chromotographic range in this application.  

It contains:

  • 0.05 mg Ribonuclease A
  • 0.025 mg Cytochrome C
  • 0.05 mg Myoglobin
  • 0.03 mg Lysozyme
  • 0.1 mg Yeast Enolase
  • 0.05 mg of alpha-cymotrypsinogen A


 It is packaged in a Waters TruView™ Vial (ideal for peptides) as a lyophilized solid.  Recommended solubilization is in 100 µl of distilled, purified Water or 50/50 mix of Mobile Phase A and B (low salt, pH 6.9).  

Store at -20 °C immediately upon arrival.

  • Protein-Pak Hi Res HIC, 2.5 µm, 4.6 mm x 35 mm with HIC Protein Standard Mix, 1/pk (176003575)
  • Protein-Pak Hi Res HIC, 2.5 µm, 4.6 mm x 100 mm with HIC Protein Standard Mix, 1/pk (176003576)
  • TruView™ LCMS Certified Vials (186005666CV)

System Performance Standards Key

Set Up

Setup standards are used upon installation of a new system, start-up of a system which has been idle for a period of time, or to re-start a system that has been moved to a new location. These standards may include multiple components within a kit which allow step by step setup and calibration, or they can be single mixtures which will enable a full system assessment and initialization.


These standards can be used for instrument calibration. Calibration is usually performed at a frequency that minimizes the risk of being out of calibration, in addition to major events suc h as when the instrument is moved and upon any major repair. Calibration can be confirmed as often as your standard operating procedures recommend. Look for solutions to calibrate your MS mass accuracy and resolution, UV detector linearity, UV and Fluorescence detector wavelength accuracy, injector accuracy and precision, and solvent system flow rate accuracy.


Your system is comprised of many interdependent components working together. An issue with any one component can produce erroneous final results. All components performing correctly will produce results within an expected variability. Some of the system components include mobile phase, column, tubing, pumping, injecting, temperature controlling, detecting, data collection rate, and integration
parameters. An issue in any one of the components listed can affect your result.
Performance Check Standards should be utilized on a routine basis. This includes anytime a column is changed, a method is changed, the instrument is moved, or confirmation of system performance is desired. Confirming the performance of your system can be done on a system basis with suitability standards or per individual instrument component. Benchmark performance should be established after
calibration and confirmed regularly.


These Mass Spectrometry solutions are used routinely and are available for every ionization mode. Use the solution to optimize the mass spectrometer setting for optimal signal either manually or through Intellistart.


This product contains hazardous material which requires special freight handling. Additional charges may apply.

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